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Description
Recombinant HEK 293 cell line expressing the human ERG (ether-a-go-go related gene) potassium channel (NM_000238).
 
Biochemical validation
Immunofluorescence of hERG HEK 293 recombinant cells or wild type HEK 293 cells with anti-hERG antibody (red). Nucleus staining is performed with Hoechst (blue).
 
Biochemical validation

Western blot analysis of recombinant or wild type HEK 293 total cell extracts with anti-hERG antibody.

 

Patch clamp

validation

Solutions:
- Extracellular solution: 118 mM NaCl, 5.6 mM KCl, 2.4 mM CaCl2, 1.2 mM MgCl2, 10 mM
HEPES, 11 mM glucose, pH 7.2
- Intracellular pipet solution: 30 mM KCl, 110 mM K-aspartate, 0.1 mM CaCl2, 1 mM MgCl2,
1 mM EGTA, 10 mM HEPES, pH=7.2
- Terfenadine: SIGMA T9652.

Recording procedure:

 

Configuration: whole-cell. Reference: Wang J et al. Am J Physiol Heart Circ Physiol. 2003 Jan;284(1):H256-67.
 

Patch clamp

validation

Currents recorded from hERG HEK 293 recombinant cell line:
   
 

I-V curves - Terfenadine (100 nM):

   
 

Maximal current inhibition of hERG with 100 nM terfenadine:

   
 
 Current stability over continuous culture:
  The hERG HEK 293 recombinant cell line was propagated over 5 months under the recommended growth conditions. There was no significant variation in expression of the hERG current over this period.
 
 
 

To know more about QT Interval Prolongation & guideline: ICH Guideline, S7A & S7B

 

     
 
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