hERG Cell Line

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Description	Recombinant HEK 293 cell line expressing the human ERG (ether-a-go-go related gene) potassium channel (NM_000238).

Biochemical validation	Immunofluorescence of hERG HEK 293 recombinant cells or wild type HEK 293 cells with anti-hERG antibody (red). Nucleus staining is performed with Hoechst (blue).

Biochemical validation	Western blot analysis of recombinant or wild type HEK 293 total cell extracts with anti-hERG antibody.

Patch clamp validation	Solutions: - Extracellular solution: 118 mM NaCl, 5.6 mM KCl, 2.4 mM CaCl2, 1.2 mM MgCl2, 10 mM HEPES, 11 mM glucose, pH 7.2 - Intracellular pipet solution: 30 mM KCl, 110 mM K-aspartate, 0.1 mM CaCl2, 1 mM MgCl2, 1 mM EGTA, 10 mM HEPES, pH=7.2 - Terfenadine: SIGMA T9652.	Recording procedure:
	Configuration: whole-cell.	Reference: Wang J et al. Am J Physiol Heart Circ Physiol. 2003 Jan;284(1):H256-67.

Patch clamp validation	Currents recorded from hERG HEK 293 recombinant cell line:


	I-V curves - Terfenadine (100 nM):


	Maximal current inhibition of hERG with 100 nM terfenadine:


	Current stability over continuous culture:
	The hERG HEK 293 recombinant cell line was propagated over 5 months under the recommended growth conditions. There was no significant variation in expression of the hERG current over this period.
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